标题: Metagenomic insight into the horizontal transfer mechanism of fluoroquinolone antibiotic resistance genes mediated by mobile genetic element in microalgae-bacteria consortia

作者: Qv, M (Qv, Mingxiang); Dai, D (Dai, Dian); Wu, QR (Wu, Qirui); Wang, W (Wang, Wei); Li, LJ (Li, Lanjing); Zhu, LD (Zhu, Liandong)

来源出版物: JOURNAL OF ENVIRONMENTAL MANAGEMENT  卷: 380  文献号: 124946  DOI: 10.1016/j.jenvman.2025.124946  Published Date: 2025 APR  

摘要: Antibiotics could accumulate in the environment with the discharge of wastewater from families, hospitals and livestock farms, which intensifies the spread of resistance genes around the world. Although microalgae-bacteria consortia (MBC) can efficiently remove antibiotics, the horizontal transfer mechanism of antibiotics resistance genes in MBC is still rarely reported. In this study, the removal efficiency of ofloxacin, norfloxacin and enrofloxacin by MBC under different antibiotic concentrations was investigated, while resistance genes in the MBC were identified and the mechanism of horizontal transfer was disclosed. The results showed that norfloxacin removal efficiency (up to 56.35 %) surpassed that of ofloxacin and enrofloxacin. The abundance of the fluoroquinolone resistance gene QnrS8 was the highest at 1331. The horizontal transfer of resistance gene QnrS8 and QnrS11 were mainly mediated by transposons. Fluoroquinolones increased the abundance of Brevundimonas (<0.10 % up to 9.63 %) and Bosea (0.96 % up to 17.67 %) involved in antibiotic removal. Arthrobacter and Acidovorax might be potential hosts which carried fluoroquinolone resistance genes. Structural equation model indicated that the key factor influencing the fluoroquinolone resistance genes abundance in MBC was transposons. These findings drew an insightful understanding of MBC application for fluoroquinolone antibiotics removal and the horizontal transfer mechanism of fluoroquinolone resistance genes.

作者关键词: Microalgae-bacteria consortia; Fluoroquinolone antibiotics; Resistance gene; Horizontal transfer; Microbial community

KeyWords Plus: WATER; FOOD; SOIL

地址: [Qv, Mingxiang; Dai, Dian; Wu, Qirui; Wang, Wei; Zhu, Liandong] Wuhan Univ, Sch Resource & Environm Sci, Hubei Key Lab Biomass Resources Chem & Environm Bi, Hubei Int Sci & Technol Cooperat Base Sustainable, Wuhan 430079, Peoples R China.

[Zhu, Liandong] Wuhan Univ, State Key Lab Water Resources Engn & Management, Wuhan 430072, Peoples R China.

[Li, Lanjing] Univ Auckland, Sch Biol Sci, Manaaki Whenua Landcare Res, Auckland, New Zealand.

通讯作者地址: Zhu, LD (通讯作者)，Wuhan Univ, Sch Resource & Environm Sci, Hubei Key Lab Biomass Resources Chem & Environm Bi, Hubei Int Sci & Technol Cooperat Base Sustainable, Wuhan 430079, Peoples R China.

电子邮件地址: ldzhu@whu.edu.cn

影响因子：8